NAD+ dependent ligases Have got a central ligase catalytic core which happens to be composed of oligonucleotide binding fold domain and nucleotidyltransferase domain.
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The impression demonstrates how ligase (yellow oval) catalyzes two DNA fragment strands. The ligase joins the two fragments of DNA to variety an extended strand of DNA by "pasting" them jointly. The system of DNA ligase is usually to sort two covalent phosphodiester bonds amongst 3' hydroxyl finishes of 1 nucleotide ("acceptor"), While using the 5' phosphate conclude of An additional ("donor").
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